Phenotypic and Genotypic Susceptibility of Anopheles gambiae s.l. to Bendiocarb and Permethrin insecticide in Kakamega County, western Kenya

Abstract

Background & objectives: Malaria vector resistance to insecticides is a major threat to the recent progresses in malaria control highlighting the need for continuous epidemiological surveillance in Kenya. Methods: WHO tube bioassay tests were used against 0.1% bendiocarb and 0.75% permethrin on Anopheles gambiae s.l. to determine phenotypic resistance. Real-time Polymerase chain reaction (RT-PCR) was used to identify G119S Acetylcholinesterase (ace1R) and L1014S mutation. Results: Anopheles arabiensis was the most prevalent species, with a frequency of 72% in Malava, 76% in Lurambi, 68% in Mumias East and 58% in Ikolomani. Possible resistance to bendiocarb was observed in all study sites. An. arabiensis exhibited possible resistance across all sites, while Anopheles gambiae sensu stricto showed phenotypic resistance in Lurambi with 87.5% mortality, but fully susceptible in Malava with 100% mortality. The G119S mutation frequency ranged from 0.6% to 2.2% in An. arabiensis while An. gambiae s.s. had a frequency of 0.0% to 1.5%. The frequency of allele was high in An. arabiensis as compared to An. gambiae s.s. across study sites with no significant deviation from Hardy-Weinberg equilibrium. Interpretation & conclusion: There is widespread insecticide resistance in malaria vectors within Kakamega County, with Anopheles arabiensis being the most prevalent species in the County. The detection of bendiocarb possible resistance in An. arabiensis across all sites and resistance in Anopheles gambiae s.s. suggests a challenge for vector control. The low frequency of the G119S ace-1R mutation indicates that resistance may be due to metabolic rather than target-site mechanisms.