Polymorphisms In Paan-Ag Promoter Influences Nf-Kb Binding And Transcription Activity In Hek293 Cells

Abstract

Activity using luciferase reporter assays. NF-KB bound both KB1 and KB2 elements in the AG1 allele. In contrast, only KB1 of the AG-2 allele bound to NF-KB; KB2 did not bind. The AG2 KB1 element bound NFKB with a stronger affinity compared to AG-1 KBl. Mutagenesis studies showed that the difference in binding was due to two nucleotide differences in the 3' end of KBl. The functional activity of the two alleles also differed; AG2 consistently showed higher luciferase activity com pared to AG 1. Mutating the last two nucleotides in the 3' end of KB1 resulted in an increase of luciferase activity to levels comparable to that of AG2. Overall, these results suggest that variations in the proximal promoter may influence

betal. While, proinflammatory and apoptotic markers were up-regulat ed in MSC-1 cells, which supports the transplant findings. Further study of the differences between primary mouse SCs and MSC-1 cells may identify novel factors important for testicular immune privilege and elucidate the mechanisms that allow SCs to prevent transplant rejection.