Immunoglobulin G bound to ovine placenta is eluted by surgical cannulation and acid perfusion in situ

Abstract

Objective: To elute placental bound immunoglobulin G (IgG) in situ. Design: Laboratory based experimentation. Setting : Biological Sciences Department, The University of Newcastle Australia and the Department of Biochemistry, University of Nairobi, Kenya. Subjects: Twelve pregnant ewes 10 to 15 days before the onset of natural parturition.

Results: Placental elutes were rich in IgG

And IgGr The relative molecular weight

of placental IgG was estimated at 158kDa by gel fl.ltration chromatography. Analysis of eluate by SUS PAGE revealed the heavy and light chains of IgG at 57 and 27kDa respectably together giving a relative molecular weight of 168kDa. Conclusion: Placental bound IgG may be crucial in immunology of pregnancy and together with the cognate antigen thereof may be useful as models for the study of maternal-fetal interaction in human pregnancy and in the development of experimental immunotherapy to immunologically compromised pregnancies in humans and livestock.