Abstract:
Zinc is one of the most abundant trace elements which plays a major role in genetic
stability and function. It is found in cell nucleus, nucleolus and chromosomes where it
is involved in stabilization of DNA, and RNA structure. In the cytoplasm, zinc is
involved in stabilizing the ribosomes and forms part of many proteins and enzymes.
We investigated the role of zinc in the management of endometriosis pain through
measurement of prostaglandins in endometriotic and endometrial cell line supernatants
and serum samples. Immortalized human endometrial stromal (T-HESC),
endometriotic stromal (22B) and epithelial (12ZVK) cell lines were used. Cells were
Stimulated with various concentrations of zinc and fetal calf serum (FCS) to optimize
the best zinc dosage and media for optimal growth. Supernatants were collected and
serum from patients who were taking zinc to relieve endometriotic pain Levels of
Prostaglandin E2 (PGE2) and PGF2 were measured in supernatants from cells treated
with Zinc and serum from patients and controls. 5μM zinc and 1% FCS concentration
were optimal for cell growth. Interestingly, endometriotic serum samples secreted
higher levels of PGE2 compared to controls. PGF2 secretion in serum obtained from
endometriotic patients and controls showed no significant difference.
This study underscores the importance of zinc in pain relief and need for further
investigation.
