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Control of Bacterial Pathogens Isolated from Water Using Actinomycetes Extracts at Egerton University, Kenya

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dc.contributor.author Waithaka, P. N.
dc.contributor.author Gathuru E. M
dc.contributor.author Muriuki, B. G.
dc.contributor.author Kamunyi, J. N
dc.date.accessioned 2021-10-16T07:25:57Z
dc.date.available 2021-10-16T07:25:57Z
dc.date.issued 2020
dc.identifier.uri http://repository.kyu.ac.ke/123456789/585
dc.description.abstract Diseases are the worst enemy to man currently. This study aimed at isolating pathogenic bacteria from shallow wells in Dundori Kenya and testing the isolates for sensitivity to antibiotic metabolites previously extracted from Actinomycetes isolates from soils of Egerton University. Water samples were collected from shallow wells randomly selected from Dundori and labelled A, B, C, D, and E. Bacterial pathogens were isolated using membrane filtration technique. The isolates were characterized using biochemical means. Antimicrobial sensitivity testing was carried out using Kirby Bauer disk diffusion method. Data analysis was carried out using the Statistical Package for Social Sciences (SPSS). Comparison of means was carried out using one-way ANOVA. Shallow wells B, D and E were highly contaminated with pathogenic bacteria. Biochemical characterization of the isolates indicated that the most common isolates were Vibrio cholera, Klebsiella pneumoniae, Proteus sp, Escherichia coli, and Staphylococcus aureus. There was no significant difference between the zones of inhibition produced by the antibiotic metabolites (F=2.149 P=1340) when tested against the test isolates. There were no significant differences between the MIC›s of the antibiotic metabolites on the bacterial pathogens (F=2.01 P=0.15). Water from some shallow wells in Dundori is highly contaminated with Klebsiella pneumoniae, Escherichia coli, Proteus sp., Vibrio cholerae and Staphylococcus aureus. The pathogens were effectively controlled using antibiotics from the Actinomycetes. There is a need to sensitize the residents of the study area on ways of preventing seepage of contaminants into the shallow wells. en_US
dc.language.iso en_US en_US
dc.publisher KyU 4th Annual International Conference en_US
dc.subject Bacteria, Egerton, control, pathogens, isolation, water. en_US
dc.title Control of Bacterial Pathogens Isolated from Water Using Actinomycetes Extracts at Egerton University, Kenya en_US
dc.type Article en_US


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