1st East African Endometriosis conferencehttp://repository.kyu.ac.ke/123456789/7062024-03-28T23:00:50Z2024-03-28T23:00:50ZEndometriosis: Socio-economic impacts and what we need to doOmwandho C.Mecha E.Sui, LNjoki A.Konrad L.Tinneberg HR.Ivo Meinhold- Heerleinhttp://repository.kyu.ac.ke/123456789/7112021-12-20T14:09:05Z2021-01-01T00:00:00ZEndometriosis: Socio-economic impacts and what we need to do
Omwandho C.; Mecha E.; Sui, L; Njoki A.; Konrad L.; Tinneberg HR.; Ivo Meinhold- Heerlein
Endometriosis is characterized by growth of uterine material outside the uterus,
excessive menstrual discharge, pelvic and lower back pain, dysmenorrhea,
dyspareunia and infertility. It has been reported to occur in 10 to 15% of women in
reproductive ages. Many theories have been advanced to explain its origin but
retrograde menstruation proposed by Sampson in 1920s proposing that eutopic
endometrial material travels to peritoneal cavity through fallopian tubes is
supported by multiple lines of scientific evidence and most widely accepted. The
disease results from a complex interplay of TGF- bettas, matrix metallo-proteinases
and a host of other reactions. The disease is managed by medical interventions but
surgery remains the gold standard for treatment. Cost of management is prohibitive
and includes ambulatory charges, hospitalization, cost of medication, time-off work
and psychosocial impacts associated with infertility. That the disease affects women
who already bear huge responsibilities at family level elevate the psychosocial
impacts to unbearable levels. There is need to form multi-institutional basic science
and clinical research teams to advance knowledge on diagnosis and management,
share data, and use research outcomes to advance awareness, advocacy and policy
changes to accommodate management of endometriosis as priority healthcare need
requiring deliberate national budgetary support.
2021-01-01T00:00:00ZMineral ions in the management of endometriosis painMecha, E.Mwaura, A.Maoga, J.Makunja, R.Omwandho, C.Tinneberg, Hans-RudolfKonrad, L.http://repository.kyu.ac.ke/123456789/7102021-12-20T14:17:22Z2021-01-01T00:00:00ZMineral ions in the management of endometriosis pain
Mecha, E.; Mwaura, A.; Maoga, J.; Makunja, R.; Omwandho, C.; Tinneberg, Hans-Rudolf; Konrad, L.
Background
A number of minerals have been implicated in management of endometriotic pain, Some of
the ions include magnesium, calcium and Zinc. Of the three minerals, zinc has been found
to play a very important role in relieving pain associated with endometriosis. Zinc metal is
one of the most abundant trace element which plays a major role in genetic stability and
function. It is normally present in many parts of the cell which include cell nucleus, nucleolus
and chromosomes whereby it is involved in stabilization of DNA structure and RNA structure.
In the cytoplasm, zinc metal is involved in stabilizing the ribosomes and it forms part of many
proteins and enzymes.
2. Objective
This study sought to investigate the role of zinc metal in the management of endometriosis
pain through measurement of prostaglandins in endometriotic and endometrial cell line
supernatants and serum samples.
3. Methods
Immortalized human endometrial stromal (T-HESC), endometriotic stromal (22B) and
epithelial (12ZVK) cell lines were used. Cell Stimulation was done with various
concentrations of zinc metal and various concentration of fetal calf serum (FCS) to optimize
the best zinc dosage and media for optimal growth. Then we collected the cell line
supernatants and serum from patients who were taking zinc metal to relieve endometriotic
associated pain and also we used serum from controls. In addition, we measured levels of
Prostaglandin E2 (PGE2) and PGF2 in supernatants from cells treated with Zinc metal and
serum from patients and controls.
4. Results
From the results, 5μM zinc concentration and 1% FCS concentration were found to be
optimal for cell growth. Interestingly, endometriotic serum samples secreted higher levels of
PGE2 in comparison with controls. But on comparison of PGE2 secretion in patients with
high pain/low pain and on pills/without pills gave contradicting results which were not
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significant. Of note, PGF2 secretion in serum obtained from endometriotic patients and
controls showed no any significant difference.
5. Conclusions
In conclusion, our study showed significant secretion of PGE2 by cell line supernatants upon
treatment with 5μM zinc concentration hence underscoring the importance of zinc in pain
relief. Although we used a small sample size, it provided us with a glimpse of the role of zinc
metal in management of endometriosis pain, thus more samples are needed to obtain a
better significant data, nevertheless, the results obtained formed a basis for further
experiments.
2021-01-01T00:00:00ZAsk the patient – What have we learned?Konrad, L.Mecha, E.Omwandho, C.Maoga, J.Riaz Muhammad, A.Ivo, Meinhold-HeerleinScheiner-Bobis, G.http://repository.kyu.ac.ke/123456789/7092021-12-20T14:18:42Z2021-01-01T00:00:00ZAsk the patient – What have we learned?
Konrad, L.; Mecha, E.; Omwandho, C.; Maoga, J.; Riaz Muhammad, A.; Ivo, Meinhold-Heerlein; Scheiner-Bobis, G.
2021-01-01T00:00:00ZExpression of membrane-type 2 and 3 matrix metalloproteinases in endometriosis and adenomyosisMaoga, J.Muhammad, A.Mwaura, A.Mecha, E.Omwandho, C.Ivo, Meinhold-HeerleinScheiner-Bobis, G.Konrad, L.http://repository.kyu.ac.ke/123456789/7082021-12-20T14:20:25Z2021-01-01T00:00:00ZExpression of membrane-type 2 and 3 matrix metalloproteinases in endometriosis and adenomyosis
Maoga, J.; Muhammad, A.; Mwaura, A.; Mecha, E.; Omwandho, C.; Ivo, Meinhold-Heerlein; Scheiner-Bobis, G.; Konrad, L.
2021-01-01T00:00:00Z